When I cannot send email from an application on a server, I use PowerShell to send an email to determine if it is the app our outside the application where the issue is.
Here is an example sending an email with the following info. You should replace this with your own names.
Mail Server: mail.example.com
Sender: [email protected] (Test Sender)
Recipient: [email protected] (Test Recipient)
Sending Server: SendingServerName
send-mailmessage -SmtpServer "mail.example.com" -to "Test Recipient